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For the quantification, the Q -value filtering was set to the classic setting, with Local Normalisation without imputing

Laser therapy works by delivering focused light energy to the skin, which stimulates collagen production , improves skin texture , and breaks down excess melanin responsible for dark pigmentation

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Calcium assay The wild-type ghrelin receptor gene was sub-cloned in the pcDNA3.0 vector with an N-terminal HA signal peptide